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Abbott Laboratories dapi ii antifade solution
Dapi Ii Antifade Solution, supplied by Abbott Laboratories, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/antifade+solution/antifade+solution/pmc13280351-69-4-8
Average 86 stars, based on 1 article reviews
dapi ii antifade solution - by Bioz Stars, 2026-09
86/100 stars

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Saline:

Article Title: Constitutive Activity of the Human TRPML2 Channel Induces Cell Degeneration
Article Snippet: .. After 3 washes with 1% NH 4 Cl (in phosphate-buffered saline), coverslips were then mounted onto glass slides with Antifade Solution (Vysis), and fluorescent images of Alexa Fluor 647 annexin V staining together with fluorescent protein emission were acquired in a confocal microscope (Olympus Fluoview 300 IX70). ..

Article Title: Heteromultimeric TRPML channel assemblies play a crucial role in the regulation of cell viability models and starvation-induced autophagy
Article Snippet: At 18 hours post-transfection, the growth medium was replaced with either fresh growth or starvation medium (Earle's balanced salt solution; Biological Industries) for 2 hours at 37°C. .. Following treatment, cells were fixed in 4% formaldehyde for 30 minutes at room temperature, washed three times with 200 mM NH 4 Cl in phosphate-buffered saline, and subsequently mounted onto slides with Antifade Solution (Vysis). ..

Staining:

Article Title: Constitutive Activity of the Human TRPML2 Channel Induces Cell Degeneration
Article Snippet: .. After 3 washes with 1% NH 4 Cl (in phosphate-buffered saline), coverslips were then mounted onto glass slides with Antifade Solution (Vysis), and fluorescent images of Alexa Fluor 647 annexin V staining together with fluorescent protein emission were acquired in a confocal microscope (Olympus Fluoview 300 IX70). ..

Microscopy:

Article Title: Constitutive Activity of the Human TRPML2 Channel Induces Cell Degeneration
Article Snippet: .. After 3 washes with 1% NH 4 Cl (in phosphate-buffered saline), coverslips were then mounted onto glass slides with Antifade Solution (Vysis), and fluorescent images of Alexa Fluor 647 annexin V staining together with fluorescent protein emission were acquired in a confocal microscope (Olympus Fluoview 300 IX70). ..

Article Title: Genetic alterations and expression of PTEN and its relationship with cancer stem cell markers to investigate pathogenesis and to evaluate prognosis in hepatocellular carcinoma.
Article Snippet: © Author(s) (or their employer(s)) 2019.. No commercial re-use.. See rights and permissions.

Fluorescence:

Article Title: Genetic alterations and expression of PTEN and its relationship with cancer stem cell markers to investigate pathogenesis and to evaluate prognosis in hepatocellular carcinoma.
Article Snippet: © Author(s) (or their employer(s)) 2019.. No commercial re-use.. See rights and permissions.

Article Title: Assessing the utility of confirmatory studies following identification of large-scale genomic imbalances by microarray.
Article Snippet: INTRODUCTION The identification of genomic imbalances that result in the loss or gain of clinically significant genetic material assists clinicians in the diagnosis, prognosis, and medical management of affected individuals.. The tools available to assess genomic imbalances have advanced greatly in the past few decades, evolving from lower-resolution techniques such as conventional karyotyping and fluorescence in situ hybridization (FISH) to much higherresolution techniques such as single-nucleotide polymorphism microarray.1 Because of its detection ability and clinical utility, the guidelines set forth by the American College of Medical Genetics and Genomics currently recommend microarray as a first-tier test for patients with intellectual disability, autism, and multiple congenital anomalies.2,3 Historically, FISH has been considered a gold standard for confirmation of copy-number changes identified by microarray.4 However, high-density microarray platforms are identifying an increasing number of aberrations that cannot be confirmed using FISH, for a variety of reasons: (i) the aberration is smaller than the detection limits of the FISH technology, (ii) the aberration is located in a region of the genome for which a clone is not readily available, or (iii) the aberration is not a result of a genomic imbalance (i.e., a region of homozygosity).. With the increasing familiarity of microarray analysis, the use of FISH or another secondary technology for confirmation of suspicious or pathogenic genomic imbalances identified by microarray has declined in some laboratories.

Concentration Assay:

Article Title: The sockeye salmon neo-Y chromosome is a fusion between linkage groups orthologous to the coho Y chromosome and the long arm of rainbow trout chromosome 2.
Article Snippet: Unlike other Pacific salmon, sockeye salmon (Oncorhynchus nerka) have an X 1 X 2 Y sex chromosome system, with females having a diploid chromosome number of 2n = 58 and males 2n = 57 in all populations examined.. To determine the origin of the sockeye Y chromosome, we mapped microsatellite loci from the rainbow trout ( O. mykiss ; OMY) genetic map, including those found on the Y chromosomes of related species, in kokanee (i.e. non-anadromous sockeye) crosses.. Results showed that 3 microsatellite loci from the long arm of rainbow trout chromosome 8 (OMY8q), linked to SEX (the sex-determining locus) in coho salmon (O. kisutch) , are also closely linked to SEX in the kokanee crosses.



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